RNA Complete BCT®
RNA Complete BCT is a blood collection tube that maintains the draw time concentration of cell-free RNA and extracellular vesicles, such as exosomes, for up to 7 days at room temperature.
RNA Complete BCT is a direct draw blood collection tube that stabilizes draw-time cell-free RNA and exosome counts for up to 7 days when stored at room temperature. It contains a preservative agent that limits the degradation of white and red blood cells, maximizing plasma yield and maintaining sample integrity during storage, shipping and handling of blood samples. The cell-free RNA and extracellular vesicles (EVs) isolated from stored plasma are suitable for many downstream applications including qualitative and quantitative real-time PCR, Droplet Digital PCR, next-generation sequencing and Nanoparticle Tracking Analysis…
…RNA Complete BCT is compatible with the following commercially available plasma nucleic acid isolation kits: Qiagen QIAamp® Circulating Nucleic Acid Kit, Norgen Plasma/Serum Circulating and Exosomal RNA Purification Kit, and Applied Biosystems MagMAX™ Cell-Free Total Nucleic Acid Isolation Kit. It is also compatible with standard low input RNA library prep kits including the New England BioLabs NEBNext® Ultra™ II Directional RNA Library Prep Kit and the Illumina TruSeq™ RNA Library Prep Kit.
Features
- Stabilizes cell-free RNA concentrations in samples for up to 7 days of room temperature storage
- Compatible with commercially available total plasma nucleic acid isolation kits and standard low input RNA sequencing library prep kits
- Isolated cell-free fRNA is suitable for downstream applications, including ddPCR and next-generation sequencing
- Isolated extracellular vesicles are suitable for Nanoparticle Tracking Analysis or indirect analysis of EV-associated cargo